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KMID : 0360220070480030431
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Journal of the Korean Ophthalmological Society
2007 Volume.48 No. 3 p.431 ~ p.437
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Evaluation of Cysticidal Effects of Contact Lens Disinfectant on Acanthamoeba Isolates
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Lee Ji-Eun
Park Jae-Sung
Lee Jong-Soo
Yu Hak-Sun
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Abstract
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Purpose: To evaluate the cysticidal effect of 5 kinds of commercially available contact lens disinfectants against 2 clinical isolates of Acanthamoeba.
Methods: Five kinds of commercially available contact lens disinfectants were soaked with cysts of Acanthamoeba ludgdunesis and castellanii at the concentration of 10(3), 10(4), and 10(5) cells/ml for 1 and 4 or 6 hours. Cysts which were not excysted in 7 days after treatment were recognized to be killed. Morphologic changes were evaluated by electron microscopic observation.
Results: Contact lens disinfectants which contain myristamidopropyl dimethylamine (MAPD) showed the best cysticidal effect. These disinfectants demonstrated a cysticidal effect on both Acanthamoeba species of all concentrations in 6-hour treatment. Contact lens disinfectants which contain polyhexamethylene biguanide (PHMB) did not demonstrate cysticidal effect, except for Acanthamoeba castellanii at the concentration of 10(3) cells/ml, in either 4- or 6-hour treatment. Separation of plasma membrane from endocyst and damage of organelles were prominent in cases showing a cysticidal effect.
Conclusions: Contact lens disinfectant which contains MAPD may be helpful in preventing the Acanthamoeba keratitis. A higher concentration of PHMB is required to be effective in preventing Acanthamoeba keratitis. J Korean Ophthalmol Soc 48(3):431-437, 2007
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KEYWORD
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Acanthamoeba, Amoebicidal effect, Contact Lens disinfectants, Cyst
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